miRNA-seq测序服务

miRNA是一类长约22 nt的非编码小RNA。miRNA通过碱基互补配对结合到靶mRNAs 3’UTRs以调控数百个基因的表达。因此,miRNA参与几乎所有生命活动,与生物体的生长、发育、疾病、衰老和死亡息息相关,可以作为疾病诊断和检测的biomarker,是经久不衰的研究热点。 miRNA测序利用高通量测序技术检测和定量样品中miRNA,并能够进行miRNA预测等分析,是miRNA研究的首选方法。

送样指南

Total RNA:2 μg
细胞:5x106
组织:50 mg
全血、血清、血浆:3 mL

结果展示


其他高级分析

经典案例

1. Ren H, Yu X, Shen G, Zhang Z, Shang Q, Zhao W, Huang J, Yu P, Zhan M, Lu Y, Liang Z, Tang J, Liang D, Yao Z, Yang Z, Jiang X. miRNA-seq analysis of human vertebrae provides insight into the mechanism underlying GIOP. Bone. 2019 Mar;120:371-386
2. Zhang H, Yue P, Tong X, Bai J, Yang J, Guo J. mRNA-seq and miRNA-seq profiling analyses reveal molecular mechanisms regulating induction of fruiting body in Ophiocordyceps sinensis. Sci Rep. 2021 Jun 21;11(1):12944
3. Li QS, Cai D. Integrated miRNA-Seq and mRNA-Seq Study to Identify miRNAs Associated With Alzheimer's Disease Using Post-mortem Brain Tissue Samples. Front Neurosci. 2021 Mar 23;15:620899. doi: 10.3389/fnins.2021.620899

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