Read before use
1, check data with precheck (windows version) tools
2, data from excel, copy and paste data into the input frame
3, data from txt, must tab-seperated, copy and paste data into the input frame
4, specieal and non-English characters such as #, <, >, %, (, ), α are not friendly
5, use point as decimal separator, not comma. e.g. 3.14, not 3,14 as pi
Required
Note: special characters are not allowed in column name
column names do not start with numbers

Optional
Figure size
figure width:
figure height:

Fontsize
ticks fontsize:
Y label fontsize:
legend fontsize:

box width:
point size:
connection line width：
connection line color:
Y label：


Box filled colors
color 1:
color 2:

add pvalue




Fontfamily


Box plot with connection lines

Introduction
Link points within box plot with connection lines by ggpubr package.
Input data instructions
Input data contain three columns: the first column is items, such as gene, the second column is dataset 1, and the third column is dataset 2.
Paper example
Multiomics data analyses to identify SLC25A17 as a novel biomarker to predict the prognosis and immune microenvironment in head and neck squamous cell carcinoma Fig 1C
Writing tips
For each patient, tumor tissues generally showed lower expression levels of SLC31A1 than normal tissues, based on data from TCGA database. Y-axis represents log2 (TPM) values of SLC31A1 expression of samples.
输入 Example
Output

1) How to plot?
1, Put data in excel according to the example format.
2, Copy and paste into input frame.
3, Input pre-checking button to check input
4, After checking pass, select parameters, submit and download

2) How to cite?
4000+ papers in (Google Scholar)
Tang D, Chen M, Huang X, Zhang G, Zeng L, Zhang G, Wu S, Wang Y. SRplot: A free online platform for data visualization and graphing. PLoS One. 2023 Nov 9;18(11):e0294236. doi: 10.1371/journal.pone.0294236. PMID: 37943830.

3) FAQs